Fig 1: Sevoflurane exposure induced cognitive impairment and suppressed Hsp70 protein levels in aged mice. (a–d) Spatial learning and memory of aged mice were determined by the Morris water maze. Escape latencies (a) and average speed of swimming (b) were recorded in the three training sessions. The duration in the target quadrant (c) and the number of times crossing the platform site during the probe trial were recorded. The Hsp70 concentrations in plasma (e) and (f) were determined in mice at 48 h after sevoflurane anesthesia with different concentrations for 3 h. Twelve mice were used for each group. Data were represented by mean ± SD. *p < .05, **p < .01, ***p < .001
Fig 2: Exogenous rHsp70 restored Hsp70 expressions in aged mice under sevoflurane anesthesia. Enzyme‐linked immunosorbent assay (ELISA) was used to measure the Hsp70 levels in serum (a) and hippocampus (b). Quantitative reverse transcription polymerase chain reaction (RT‐qPCR) was used to measure the mRNA expression of Hsp70 in the hippocampus (c). Hippocampal Hsp70 protein expression was determined by Western blot analysis (d). Twelve mice were used for each group. For the experiments of RT‐qPCR and Western blotting, the hippocampal homogenate from 12 mice in each group was mixed, and the experiments were repeated for four times. Data were represented by mean ± SD. *p < .05, **p < .01, ***p < .001
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